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At Synbio Technologies, we offer advanced
Protein
S
tructural
Analysis
Services
to decipher complex biomacromolecules with speed and precision. Backed by
750+ successful projects
for
350+ global clients
, we combine
X-ray, CryoEM-SPA
, and
MicroED
platforms to tackle diverse structural challenges.
With weekly synchrotron access and a
one-stop service
from gene synthesis to structure determination, we deliver reliable results in just 1–2 months.
Case Overview
IL5RA
expression was challenging due to its tendency to form inclusion bodies and the small size of its cocrystals (<10 μm). Using Cryo-FIB for lamella preparation and MicroED for data collection, we resolved the structure of IL5RA in complex with AF17121 peptide.
Challenges & Solution
Inclusion Body Refolding
- Purified IL5RA inclusion bodies and refolded the protein to obtain soluble IL5RA with >90% purity
MicroED Structural Determination
- Employed Cryo-FIB to prepare 200 nm-thick lamellae and obtained high-quality diffraction data at 3 Å resolutions.
High-Resolution Insights
- Clearly resolved the electron density of the AF17121 peptide bound to IL5RA.
We overcame complex structural challenges to deliver fast, accurate results
!
Contact Us
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Synbio Technologies LLC
9 Deer Prk Dr., Suite J-25, Monmouth Junction, NJ 08852, USA